FastQCFastQC Report
Fri 10 Feb 2017
SRR3559390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4240311
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT997202.3517142964277857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463931.0940942775187952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305480.7204188560697553No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179320.42289350946192394No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148270.3496677484269432No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105040.2477176791985305No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79180.18673158643316493No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76520.18045846165528895No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76070.17939721874173853No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT52620.124094671357832No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC49940.11777438022824269No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT48940.11541606264257505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA392650.056.53741
TAACGCA7750.047.6418344
TATCAAC503400.044.22062
ATCAACG503550.044.0891573
TCAACGC515700.043.639354
CAACGCA518600.043.4414025
AACGCAG534000.043.013566
TATCACG6750.040.5837862
ACGCAGA584650.039.185477
CGCAGAG594750.038.6399968
GCAGAGT621750.036.847269
CGCATAG9550.036.3210261
TACAACG8700.036.2789882
GGTATCA207250.033.848311
GTATAAC12400.033.2782821
ACAACGC10000.031.5627163
CATGGGT49350.031.4957454
CATGGGG134450.030.5623534
AGTACTT408100.028.97182512-13
AGAGTAC608800.028.86056510-11
Produced by FastQC (version 0.11.5)