FastQCFastQC Report
Fri 10 Feb 2017
SRR3559381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4373810
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1227852.8072778652936456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT564051.2896079162103522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355210.8121294706445868No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215520.49275117117570266No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162180.37079799991311924No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114780.26242566549530044No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA102230.23373214657243913No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97700.22337504372617922No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85600.19571037607943645No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT76930.17588784149288605No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT72650.16610232268891426No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC69750.1594719477983726No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC55510.1269145207496439No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG51800.11843221356208888No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA50200.11477407569144521No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT49760.11376808777701822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA491000.057.308031
TATCAAC619350.045.470712
CGCATAG10350.045.0704151
ATCAACG632450.044.4441223
TCAACGC649800.043.834824
CAACGCA652200.043.732255
AACGCAG672300.043.2477576
TACAACG10350.042.0030752
TAACGCA7450.040.7673454
ACGCAGA737950.039.3600357
CGCAGAG745850.039.0392468
ACAACGC10800.038.5987173
GCAGAGT773350.037.481889
TCACGCA8750.035.3910834
GACCGTT8950.034.5460327
TATCACG8650.033.734832
GGTATCA254450.033.1401141
GTACAAC14200.032.850621
GTACTGG39250.032.6070441
TACTGGT32850.032.4501152