FastQCFastQC Report
Fri 10 Feb 2017
SRR3559380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7851993
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2233092.843978592441435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1138091.4494281897602304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT702210.8943079801523002No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427360.5442694612692599No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299380.3812789950271224No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239290.30475065375121957No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187920.23932777321630316No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183630.23386419218662063No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC134040.17070825203231843No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT126880.1615895480293984No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA109780.13981163763136314No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC103530.13185187505898185No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA99840.12715243123624792No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT90920.11579225809294533No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT84070.10706835831361541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA728900.065.474311
TATCAAC969800.049.2740942
ATCAACG987350.048.2896773
CAACGCA1021600.047.189795
TCAACGC1020250.047.1877864
AACGCAG1050800.046.5643166
GGTATCA330800.044.051091
CGCATAG17650.043.7209741
ACGCAGA1165350.041.8698467
CGCAGAG1181550.041.336048
GCAGAGT1233350.039.464969
TATCACG14800.035.812792
TAACGCA14200.034.809654
TACTGGT59150.034.3328252
TCACGCA17100.033.0854724
GTATAAC17550.032.7219351
ATAGTAC24350.032.2838134
GTACTGG70350.032.2270051
AGAGTAC1216800.031.3573310-11
GGACCGT21850.031.2948346
Produced by FastQC (version 0.11.5)