FastQCFastQC Report
Fri 10 Feb 2017
SRR3559371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194029
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570332.599464273261657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263331.2002120300141885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185850.8470717570278242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96550.44005799376398397No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79720.36334980075468465No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57650.26275860528735034No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44950.20487422910089154No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40860.18623272527391388No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33280.15168441255790147No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC29940.13646127740335245No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT23420.1067442590777059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA205900.063.4931871
TATCAAC264450.049.0593832
ATCAACG269300.048.1095163
TCAACGC277850.047.4005244
CAACGCA280550.047.035215
CGCATAG5750.046.7393231
AACGCAG288700.046.408066
TAACGCA4350.045.1682364
TACAACG3800.042.30472
ACGCAGA319350.041.916737
CGCAGAG323850.041.517998
GGTATCA96000.041.0591961
GCAGAGT337750.039.8093349
ATAGTAC7700.034.7960744
GTACAAC6150.033.9883961
TATCACG3350.033.7689252
CATGGGT28650.033.4587484
CATGGGG75350.033.424554
GTACTGG15650.032.8187371
GTATAAC6950.032.654011