FastQCFastQC Report
Fri 10 Feb 2017
SRR3559370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6727784
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1703842.5325426618928315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT792381.1777726514406526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530720.7888481556482788No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286620.42602437890396005No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191150.284120298749187No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147420.21912118462780614No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133360.1982227729070969No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115900.17227069121125174No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC79920.11879097188613665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA532200.068.561
TATCAAC703850.051.5631182
ATCAACG713550.050.7533073
GGTATCA227050.050.3578341
TCAACGC736300.049.71084
CAACGCA740100.049.5017245
AACGCAG762450.048.9323926
ACGCAGA844700.044.139587
CGCAGAG858350.043.5277528
GCAGAGT898350.041.6359949
TATCACG10650.036.3420452
TACAACG12550.035.5846822
CGCATAG15750.034.8942531
TAACGCA10650.033.546254
AGAGTAC886750.032.1777310-11
CATGGGG206700.030.9101854
AGTACTT665850.029.81809412-13
ACATGGG475700.029.2903843
ACAACGC15250.029.2842253
CATGGGT73650.029.186154
Produced by FastQC (version 0.11.5)