FastQCFastQC Report
Fri 10 Feb 2017
SRR3559363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4185419
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940922.248090334563875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438971.048807777668138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290260.6935028488187205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156880.37482507725033026No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130760.3124179442966164No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96940.23161360905562858No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70490.16841802457531732No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68560.16380677776824734No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA66810.15962559543023053No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT51700.12352407250026819No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC51310.12259226615065302No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT45620.10899745043447262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA370150.057.354841
TATCAAC472350.044.7902872
ATCAACG475700.044.3497283
CAACGCA492200.043.7444575
TCAACGC490350.043.7166944
TATCACG7150.043.2939952
AACGCAG507550.043.2185676
ACGCAGA557500.039.431717
CGCATAG9950.039.0120241
CGCAGAG566700.038.9805378
GCAGAGT593400.037.2165839
TACAACG7950.035.942182
GGTATCA192100.035.159561
TCACGCA10100.032.4169164
TAACGCA7900.032.4019934
CATGGGG129900.029.100134
AGAGTAC579100.028.95664810-11
GTATCAC14050.028.902861
ATCACGC11600.028.7382563
AGTACTT394100.028.67776112-13