FastQCFastQC Report
Fri 10 Feb 2017
SRR3559362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993202
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT595452.9874041868310384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266361.336342227230356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187530.9408479421553863No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104630.5249342515209197No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101130.507374566150345No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64480.32349957505561405No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52930.2655526133327179No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44400.22275715155814615No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42690.21417799099137969No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36890.1850790838058561No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT30590.15347165013882186No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30010.1505617594202695No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC27340.1371662280090026No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.13064405915707492No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23010.11544238867912034No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC20880.10475606586788494No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20290.10179600461970237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG4850.060.1538472
TAACGCA5950.053.0356564
TATCACG3300.052.3230552
CGCATAG5550.051.6444781
GTATCAA266050.051.0615231
ACAACGC5800.050.3010643
TCACGCA4050.047.0439764
GTACAAC6750.046.886491
GTATAAC7300.045.8079451
TATCAAC327700.041.2074432
ATCAACG333550.040.3954663
TCAACGC344550.039.8661584
CAACGCA348300.039.6454055
AACGCAG359000.039.5078126
GTACTGG15400.038.3875241
GGTTTCA11700.037.7677841
ACGCAGA391800.036.0637057
CGCAGAG395450.035.926428
AAACGCA8750.035.3562745
TACTGGT14100.035.0483862