FastQCFastQC Report
Fri 10 Feb 2017
SRR3559361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4189056
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1097222.6192535979466496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT518341.2373670822256853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355990.8498095991077703No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189170.45158145415100687No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145690.347787186420998No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109740.26196832890274085No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84660.202098038316986No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79080.18877761481345678No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43670.1042478305374767No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC42410.10123999297216364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA355750.063.9241451
TATCAAC473550.047.9001462
ATCAACG475600.047.5935333
CAACGCA493450.046.997035
TCAACGC492050.046.921974
AACGCAG508950.046.606116
GGTATCA164900.045.631291
CGCATAG10100.044.9371031
TATCACG5700.044.9129182
ACGCAGA557950.042.470447
CGCAGAG565350.041.935588
GCAGAGT592950.039.983619
TAACGCA6950.034.265174
TACAACG8050.033.2808462
ATAGTAC13950.032.862024
AGAGTAC582700.031.4319310-11
AGTACTT437450.030.06597512-13
AGATTAC18350.029.5245322
CATGGGG114650.028.7682464
ACAACGC9350.028.653563
Produced by FastQC (version 0.11.5)