FastQCFastQC Report
Fri 10 Feb 2017
SRR3559358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819183
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT557011.4584532869988163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254860.6673154965342064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183020.4792124388907261No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78320.20507003723047573No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC44060.1153649877473795No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42870.11224913810100223No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40140.10510101244166618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA243550.061.8996351
TATCAAC320150.046.9511762
ATCAACG319250.046.859773
TCAACGC326500.046.8038184
CAACGCA329000.046.4744455
AACGCAG341450.045.8079036
ACGCAGA376350.041.5125857
CGCAGAG384150.040.6851778
GGTATCA117100.040.4325521
GCAGAGT400850.039.0050139
TATCACG4850.038.050342
CATGGGG114500.033.6385574
ACATGGG261350.032.2081263
GTACATG275050.032.061491
TACATGG278400.031.5827452
AGAGTAC394650.030.24851210-11
CATGGGT46600.029.5096914
GTACTGG28350.028.6418271
AGTACTT265300.028.40359712-13
GAGTACT242550.027.96462212-13
Produced by FastQC (version 0.11.5)