FastQCFastQC Report
Fri 10 Feb 2017
SRR3559353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274596
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375852.948777494986647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171851.348270353900373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127030.99662952025583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70660.5543717381821378No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64430.5054935053930814No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41950.32912389494396654No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.22760153021035684No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26080.20461385411534322No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20610.16169829498915736No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.12772674635727713No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT13510.10599436998076253No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC13150.10316994561413968No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13030.10222847082526543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3950.061.9728361
GTATCAA140400.060.2158361
TAACGCA2750.058.4428064
TATCACG2100.051.0235022
TACAACG2950.050.4469642
TATCAAC173750.048.204362
ATCAACG175950.047.329123
ACAACGC3400.047.2699173
TCAACGC180650.047.1192174
CAACGCA182450.046.759165
AACGCAG188600.046.3699956
ATAGTAC5650.044.2487344
GGTATCA69200.043.571231
ACGCAGA209850.041.4476367
CGCAGAG211600.041.3578958
GTATAAC4250.040.7402461
GCAGAGT221750.039.464859
TCACGCA2950.038.338194
ATAACGC4900.037.6587263
AGATTAC7800.035.8690832
Produced by FastQC (version 0.11.5)