FastQCFastQC Report
Fri 10 Feb 2017
SRR3559352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4044212
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1313783.2485438448825135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT588141.454275888603268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402800.9959913080718814No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224580.5553121349721528No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144170.35648477379524124No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111020.2745157771155419No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101080.24993744145954763No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92570.2288950233073835No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC71760.17743876928311375No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59830.1479398211567544No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT59010.145912232098614No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA49970.12355929906740794No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45950.11361916734335391No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC44570.11020688331867864No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT42680.10553353780662339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA432050.066.188821
TATCAAC557450.051.1665882
ATCAACG570050.049.8789833
TCAACGC588800.048.816394
CAACGCA591900.048.617565
AACGCAG609050.048.0300146
GGTATCA192250.044.9755061
ACGCAGA671950.043.4720347
CGCAGAG677450.043.171798
GCAGAGT707550.041.394079
CGCATAG10250.041.3626481
TACAACG7000.038.271962
TAACGCA7800.035.109894
CATGGGT67900.034.282564
TATCACG6750.033.5155262
TCACGCA7250.032.8464364
AGAGTAC702200.031.52683410-11
ACAACGC8700.031.477833
ACGGTAC11100.031.1079393
CATGGGG134450.031.0401234
Produced by FastQC (version 0.11.5)