FastQCFastQC Report
Fri 10 Feb 2017
SRR3559351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3415806
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT883402.586212448833453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416011.2178970351360703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275720.8071886986556028No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160140.46882053606088875No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134580.39399193045506686No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97630.2858183398003282No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68960.2018850016657855No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68880.2016507963274261No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50700.148427633185257No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43190.1264416070467702No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38800.11358958910429925No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT35300.10334310555107637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA306750.061.326381
TATCAAC395250.047.353912
ATCAACG400250.046.6284943
TCAACGC412550.046.0030754
CAACGCA413000.045.8952185
TACAACG6400.045.578632
AACGCAG427050.045.5554926
TATCACG5850.043.758032
ACGCAGA472300.041.190927
ACAACGC6850.040.8462753
CGCAGAG478900.040.75998
CGCATAG8500.040.0476721
GGTATCA149800.040.02611
GCAGAGT500550.039.008829
TAACGCA6650.036.7034874
ATAGTAC11150.032.0347634
AGATTAC15350.031.4138952
TAGTACT12500.030.4608235
AGAGTAC491800.030.31008510-11
GTACAAC11000.029.8601071