FastQCFastQC Report
Fri 10 Feb 2017
SRR3559349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4093007
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT996222.433956257587637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461701.1280215254945813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302590.7392853225025025No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173600.4241380481391798No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138120.33745361295497417No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101800.2487168968926757No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76880.18783256417592248No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73470.17950128108747432No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA53060.12963574213286222No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC52360.12792550806778488No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT43770.1069384928977644No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA339450.063.828341
TATCAAC439400.049.100842
ATCAACG443900.048.5628473
TCAACGC458150.047.884024
CAACGCA463600.047.5448045
CGCATAG9700.047.406841
AACGCAG479250.047.0970236
ACGCAGA527150.042.7046557
GGTATCA157650.042.616781
CGCAGAG534350.042.2405748
GCAGAGT562900.040.1404279
TAACGCA8250.038.245714
TATCACG6300.036.854022
CATGGGG126550.031.8954224
TACAACG8400.031.1841682
ATAGTAC15600.030.9115814
AGAGTAC551950.030.83805810-11
AGTACTT405350.029.54778712-13
ACATGGG306900.029.2332533
GTACATG323800.029.067081
Produced by FastQC (version 0.11.5)