FastQCFastQC Report
Fri 10 Feb 2017
SRR3559346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5021774
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1574463.1352665412660943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT725701.4451068486953016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463560.9231000837552625No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272800.5432343231694616No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204430.40708721658919733No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150170.29903775040453834No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126490.2518830994783915No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116260.23151181235953666No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC78410.156140041347938No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA75170.1496881380962186No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT65160.1297549431734682No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62400.12425887744052201No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA57030.11356544519924633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA516100.066.663351
TATCAAC675850.050.765382
ATCAACG687050.049.7895283
TCAACGC708850.048.85464
CAACGCA715850.048.435055
AACGCAG734400.047.859716
TACAACG10750.043.7512
ACGCAGA813850.043.114437
CGCAGAG821700.042.8039058
GGTATCA227050.042.5242081
GCAGAGT854650.041.21639
CATGGGT95750.038.486874
ACAACGC12700.037.032593
TATCACG9050.035.5234032
CGCATAG11900.034.6218221
GTACAAC15450.034.3961071
GTACTGG36150.032.5391121
CATGGGG160450.032.3547064
AGAGTAC848050.031.43243810-11
ATGGGTG51950.031.1491055
Produced by FastQC (version 0.11.5)