FastQCFastQC Report
Fri 10 Feb 2017
SRR3559345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371949
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT708662.9876696337062896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333631.4065648123125751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222550.9382579473673338No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130680.5509393330126406No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114680.48348425703925335No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82150.34633965570086034No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55590.23436422958503744No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53060.22369789569674559No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31550.13301297793502306No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC27790.11716103508127704No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT25810.10881346942957038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA199000.069.592181
GGTATCA82750.054.270341
CGCATAG7250.053.5411951
TATCAAC268000.051.3612142
TACAACG5350.051.190142
ATCAACG269900.050.8882873
TCAACGC282600.049.9285934
CAACGCA287050.049.245775
AACGCAG296900.048.874426
ACGCAGA328850.044.0897567
CGCAGAG336000.043.364028
ACAACGC6200.043.210963
GCAGAGT352500.041.300469
AGATTAC12350.038.0839842
TATCACG3950.037.681242
ATAGTAC10850.037.312324
TAACGCA4950.037.284614
CATGGGG65750.035.8568844
GTACAAC8350.035.7598951
CATGGGT34850.035.5331464