FastQCFastQC Report
Fri 10 Feb 2017
SRR3559341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550955
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT699131.9688506331395357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325350.9162323938208172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218700.6158906547675203No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124760.3513421037439224No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96310.2712228118914489No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68770.19366621092072414No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53440.15049472606664968No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47890.13486512783180862No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46790.1317673696231014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA276200.058.4477271
CGCATAG9750.047.183711
TATCAAC356800.044.975362
TATCACG4900.044.962082
ATCAACG361900.044.3251043
TCAACGC370500.043.890874
CAACGCA375250.043.6468625
AACGCAG393450.043.0341156
TACAACG7100.042.771312
ACAACGC7700.040.985093
ACGCAGA435250.038.9422657
CGCAGAG441500.038.4448858
GTATAAC10300.037.7035371
GCAGAGT460850.036.8306779
TAACGCA8550.036.2141234
GGTATCA141650.035.5141561
ATAGTAC14750.031.891564
TAGTACT16000.030.1184485
ACATGGG271700.029.5859053
AGAGTAC452900.029.06415410-11