FastQCFastQC Report
Fri 10 Feb 2017
SRR3559340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081117
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463362.2264966361814356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223811.0754320876721493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151920.7299925953226081No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81850.3932984065768527No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70500.33876038684994647No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51280.24640613670447167No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46580.22382211091447526No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35700.17154249376656863No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35370.16995680684939865No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT33640.16164396331393188No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC31920.15337917089716724No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30790.14794939448382768No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT25880.12435629520108672No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA25000.12012779675530016No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23200.11147859538891854No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21850.10499169436413235No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21610.10383846751528146No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC21460.10311770073474966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA5700.057.443324
GTATCAA208800.051.077631
CGCATAG6500.049.6089671
TATCACG3250.049.4574932
TACAACG4000.047.6257362
GTATAAC6950.042.9600681
TATCAAC263500.040.14752
TCAACGC275900.039.4651454
ATCAACG268850.039.3264353
ATAGTAC8350.039.2128034
AACGCAG289250.039.1001666
CAACGCA279700.039.0526125
CATGGGT29600.038.816584
AGATTAC9400.037.3659362
ACAACGC5100.037.3535163
ACGCAGA317600.035.497577
CGCAGAG320600.035.2581868
GCAGAGT330500.034.1300359
CATGGGG61350.034.0599674
AAACGCA8500.033.596365