FastQCFastQC Report
Fri 10 Feb 2017
SRR3559333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6465909
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1434202.2180949345250602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT718251.1108260261627563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463290.7165117851179161No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253900.39267487371071874No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224280.3468653827327295No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154860.23950228807736082No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112190.17351002001420063No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109620.16953532751543518No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA87780.13575817414071245No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT86210.1333300545986651No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC70880.10962109117217704No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC65090.1006664337527794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA518750.057.7960321
TATCAAC673650.044.3324472
ATCAACG679800.043.7037123
TCAACGC698650.043.1700944
CAACGCA704900.042.835335
AACGCAG727650.042.305566
ACGCAGA792200.038.8358887
CGCATAG13250.038.7414171
CGCAGAG806450.038.3341038
GGTATCA268150.037.5962751
GCAGAGT846200.036.49689
TAACGCA10700.032.265324
TATCACG8850.031.613062
TACAACG13200.029.3123972
AGAGTAC828550.029.29666710-11
GTACTGG53450.029.2581271
AGTACTT570500.029.00682412-13
TACTGGT47000.028.496832
ACAACGC13800.028.4692973
CATGGGG186750.027.2838044
Produced by FastQC (version 0.11.5)