FastQCFastQC Report
Fri 10 Feb 2017
SRR3559330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6609131
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1485962.248343995602448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT711751.0769191895273371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT459980.6959765209677339No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268760.406649527751833No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228890.34632389643963785No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158860.24036442915112438No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT140450.21250902728361717No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119650.18103741626546666No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118830.17979670852340499No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC116260.17590814889279696No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA96900.14661534171436455No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC93610.14163738016389749No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT89210.13497992398698105No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA75700.1145385074074035No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT74450.1126471846298704No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT71790.10862244975928001No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA70790.10710939153725353No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT69530.10520293817750019No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT66710.10093611399138555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA571500.059.719321
TATCAAC732350.046.5610122
ATCAACG740350.045.9292563
TCAACGC762250.045.257094
CAACGCA767100.045.0311625
AACGCAG788550.044.5833666
ACGCAGA864050.040.6257367
CGCAGAG877350.040.070918
GCAGAGT921650.038.078589
CGCATAG11450.038.0476951
GGTATCA284550.037.6248551
TACAACG12900.035.988792
GTACTGG61100.035.747931
TACTGGT58500.034.592772
TAACGCA10100.033.5899354
TATCACG9450.032.7516982
ACAACGC13900.032.115063
ACGGTAC20700.030.766333
GGTTCAC64750.029.862886
AGAGTAC904650.029.44580310-11
Produced by FastQC (version 0.11.5)