FastQCFastQC Report
Fri 10 Feb 2017
SRR3559329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3741307
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT867002.3173719772261405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421551.1267452791230446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278440.7442318954311956No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156760.41899795980388677No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138680.37067260184742923No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96680.2584123676565435No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT74040.19789875570221852No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68470.183010910358332No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67910.18151410723578684No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC63260.16908529559322452No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54940.14684707777255382No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT51190.13682384257693903No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC47410.12672042149975932No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA44330.11848800432576104No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT40500.10825094011263979No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT39770.10629975032789343No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT38450.10277157153903702No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC37860.10119458253492696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA319300.061.311181
TATCAAC415300.047.182642
ATCAACG419250.046.5251433
TCAACGC430050.046.006654
CAACGCA432400.045.8890155
AACGCAG443750.045.5599866
ACGCAGA490750.041.1238827
CGCAGAG498900.040.5594188
GCAGAGT521700.038.7505539
TATCACG5700.038.63772
GGTATCA156400.038.505181
CGCATAG8500.037.9174921
TAACGCA5950.036.0133974
GTACTGG33850.033.853791
TACTGGT30450.032.6447642
CATGGGT40250.031.6465534
TACAACG7050.031.238992
AGAGTAC515500.030.03567710-11
ATAGTAC11050.029.6264064
AGTACTT363550.029.57122612-13
Produced by FastQC (version 0.11.5)