FastQCFastQC Report
Fri 10 Feb 2017
SRR3559327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543663
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT573612.2550550131837435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262041.0301679113939228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185090.7276514223778858No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101050.3972617441854522No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92380.3631770403547954No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60470.23772803236906775No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51430.20218873333456513No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT50890.20006581060462805No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42740.1680254027361329No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41690.16389749742792187No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC38840.15269318301992046No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT37580.14773969665006725No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC33520.13177846279165123No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT31620.12430891985298366No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26710.10500604836411113No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA26310.10343351300860215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA255500.053.03461
CGCATAG5450.048.192621
TATCACG4100.046.4715232
TATCAAC321650.042.168772
ATCAACG323200.041.7270473
TCAACGC331150.041.3709534
TAACGCA4750.041.3641434
TACAACG5350.041.178332
CAACGCA334100.041.1166845
AACGCAG344450.040.9520846
ACAACGC5500.040.0552833
ACGCAGA376150.037.45347
CGCAGAG381700.037.04918
TACTGGT21450.036.3634222
GCAGAGT394550.035.7211239
GTACTGG24350.034.8107761
TCACGCA5750.033.1349144
GGTTCAC22850.032.8061266
ACTGGTT25450.030.6481533
AGATTAC9600.030.3910452
Produced by FastQC (version 0.11.5)