FastQCFastQC Report
Fri 10 Feb 2017
SRR3559312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4898449
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT873421.7830541871518926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401660.8199738325335224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273330.5579929483801913No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129180.26371612728845395No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT96530.19706237627461265No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC79120.1615205139422703No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC68700.14024847456817455No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67890.13859488993352792No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64980.13265423402387164No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA62330.12724435836731177No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT62100.12677482198957263No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA57480.11734326518455128No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55940.11419941291621082No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA51140.10440039285904579No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50420.10293053985047104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA386350.060.358841
TATCAAC499150.046.6247022
ATCAACG502650.046.1105883
CAACGCA516500.045.477385
TCAACGC515150.045.4645164
AACGCAG533200.044.789466
ACGCAGA588050.040.6218727
CGCAGAG594650.040.211038
GCAGAGT625900.038.097649
GGTATCA190100.037.4791261
GTACTGG45450.033.479081
TACTGGT43800.030.8476542
CATGGGG163100.029.9241264
GGTTCAC42950.029.782696
AGAGTAC615150.029.6528510-11
AGTACTT395350.029.2919312-13
ACATGGG390000.028.6616633
TAACGCA7500.028.569514
GTACATG418850.028.0513951
TACATGG420200.027.9757332
Produced by FastQC (version 0.11.5)