FastQCFastQC Report
Fri 10 Feb 2017
SRR3559290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5457990
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1381762.53162794362027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT669451.226550433401307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441490.8088875208639077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270540.49567697998713817No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237570.4352701269148533No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167600.30707275022489966No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT125340.22964497919563798No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116530.2135035058693768No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110340.20216233448577225No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC84870.15549680376842023No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT76450.1400698792046156No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC69870.1280141590585545No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA63430.11621494359645218No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT57350.1050753116073866No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT55980.10256523005721886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA495700.063.4432141
TATCAAC632800.049.5735632
AACGCAG657000.048.9523936
ATCAACG643350.048.5385673
TCAACGC659300.047.878944
CAACGCA667500.047.487165
ACGCAGA732650.043.8571977
CGCAGAG742350.043.340238
CGCATAG11650.042.7669141
GGTATCA231550.041.63481
GCAGAGT779700.041.241219
GTACTGG50650.039.3472481
TACTGGT46900.039.2185552
GGTTCAC52300.033.8982936
CATGGGG186750.033.69154
CATGGGT71600.032.839014
ACATGGG452050.032.524943
GTACATG468850.032.4947661
GGACCGT13750.032.450536
TACATGG474300.032.203982
Produced by FastQC (version 0.11.5)