FastQCFastQC Report
Fri 10 Feb 2017
SRR3559289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879485
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT653152.2682875583654716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341711.186705261531142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230230.7995526977914453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148990.5174189134515373No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137310.4768561044770158No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94770.3291213532975515No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT80930.2810572029373308No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57960.20128599384959464No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54520.18933941312422187No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT53360.18531091497264268No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC45770.15895203482567197No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC43270.15026992674037198No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA39870.13846225974436402No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT36950.12832155750073362No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT34660.12036874649459886No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA33100.11495111104937168No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA32900.11425654240254768No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC32310.11220756489441687No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT32110.1115129962475929No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC31790.11040168641267449No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29580.10272670286526932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG6050.051.1645432
GTATCAA292250.050.2209631
CGCATAG8000.048.062471
TAACGCA6400.044.645994
TACTGGT26850.044.119442
TATCACG3400.043.7705762
ACAACGC7300.043.218953
GTACTGG29400.042.2998731
AACGCAG372150.041.020726
TATCAAC361850.040.6175462
ATCAACG367850.039.7932053
TCAACGC376600.039.437684
CAACGCA382300.039.1068535
GGTTCAC29700.038.460026
CATGGGT39000.037.243154
ACGCAGA414750.036.864767
CGCAGAG418800.036.664528
ACTGGTT32500.035.5336233
CATGGGG88200.035.1633534
GCAGAGT437050.035.1471259
Produced by FastQC (version 0.11.5)