FastQCFastQC Report
Fri 10 Feb 2017
SRR3559286.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559286.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8874634
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2939963.312767602585075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360951.5335280305644154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT929751.0476488382506817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479700.5405293333787061No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316400.35652174500942796No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT243930.27486203938100434No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240720.27124498880742576No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231190.26050651778991674No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199840.22518111732833151No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC162630.1832526276576589No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC141620.1595784119097193No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT118560.13359424174563142No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA109060.12288957493909045No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT108630.12240504791521543No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT103420.11653438327710192No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT102940.11599351590161353No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC99380.11198208286674133No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA99080.11164404075706108No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC95210.10728329754218598No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG91560.1031704518744097No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA90120.10154784974794455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA980450.069.365051
TATCAAC1267250.053.7616842
GGTATCA408900.053.373441
AACGCAG1310300.052.4696
ATCAACG1303200.052.1695673
TCAACGC1329950.051.4470254
CAACGCA1342100.051.0263185
ACGCAGA1453800.047.1999247
CGCAGAG1470350.046.688888
GCAGAGT1535100.044.64989
GTACTGG90400.036.957521
TACTGGT86150.035.519362
AGAGTAC1523800.033.75851410-11
ACGGTAC31300.032.714953
AGTACTT1072700.032.04886212-13
GGTTCAC95350.031.5715456
CATGGGT122400.030.3991054
ACTGGTT100600.030.3585873
GTACATG850800.029.959851
CGCATAG13650.029.934551
Produced by FastQC (version 0.11.5)