FastQCFastQC Report
Fri 10 Feb 2017
SRR3559283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117966
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436142.0592398555973044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218261.030517014909588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154490.7294262514129122No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101180.47772249412880097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88040.41568183814093335No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60870.287398381277131No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT43530.20552737862647463No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37110.17521527729906902No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.16402529596792392No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32390.15292974485898264No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT28460.1343742061959446No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC25990.12271207375378075No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC24160.11407170842213708No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA21370.10089869242471314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5800.053.80081
GTATCAA205800.050.8819121
TATCACG3300.048.7013172
TAACGCA4600.046.586074
TACAACG4100.043.5540242
TACTGGT18950.043.347172
GTACTGG22450.042.500511
AACGCAG259900.041.891956
TATCAAC251500.041.370842
ATCAACG255000.040.73493
GTATAAC6050.040.6669731
TCAACGC263400.040.2946134
CAACGCA267800.039.8119245
GGTTCAC20400.039.3721736
ACTGGTT21000.038.834053
ACGCAGA287200.037.909887
ACAACGC4900.037.6597143
CGCAGAG290700.037.5557868
ATAGTAC8450.037.336244
CATGGGG75200.036.254264