FastQCFastQC Report
Fri 10 Feb 2017
SRR3559272.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559272.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856261
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1033203.617316484733013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521801.8268638615308612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352891.2354963359440891No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182330.6383520273532426No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137380.480978453999827No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102020.3571802436822125No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86210.301828159261356No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76230.2668873747882284No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT59460.20817425298318326No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC45790.16031448106458057No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC37520.13136054443203896No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT34520.12085730260644949No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA31930.11178950383035724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA369550.062.6367721
AACGCAG481350.049.3884356
TATCAAC471550.049.266862
CGCATAG7300.048.529671
ATCAACG483850.047.8544773
TCAACGC498050.047.099764
CAACGCA501400.046.9151425
ACGCAGA535950.044.345887
GGTATCA173100.044.0192641
CGCAGAG541400.044.0093548
TAACGCA4600.044.0064664
TCACGCA5450.042.6052864
GCAGAGT566800.041.9846849
TATCACG5400.041.897222
ACGGTAC8300.038.018343
GTACTGG24800.037.2861251
TACTGGT23000.037.2760622
ATAGTAC9500.036.3496134
GTATAAC6700.035.847871
CATGGGG83700.034.1437454
Produced by FastQC (version 0.11.5)