FastQCFastQC Report
Fri 10 Feb 2017
SRR3559265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357529
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT787742.346189712732191No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389851.161121765441192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255770.7617804641449113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179440.5344406556131012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152540.45432221136436945No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111000.3306002718070343No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71070.21167352538131465No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT68420.2037808161895251No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66300.19746664883609344No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT44480.13247837918898095No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC44260.13182313540702106No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41380.12324539862500071No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39650.11809279979413431No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT35790.10659624980156539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6700.059.5299382
GTATCAA333650.054.8911321
CGCATAG8500.051.586951
AACGCAG425600.045.0528566
TCACGCA9250.044.4067764
TATCAAC418050.043.6880382
ATCAACG423350.043.0573733
TACAACG6500.043.0447242
TCAACGC437000.042.5434234
CAACGCA444250.041.9294625
GTATCAC10500.041.7608641
ATCACGC9500.041.358263
ACGCAGA469850.040.7465177
CGCAGAG474600.040.501678
TAACGCA8250.040.4088024
GCAGAGT499050.038.4339189
CATGGGT56750.038.078754
AGATTAC16800.037.91492
GTACTGG30000.037.8422051
TACTGGT28150.036.796482