FastQCFastQC Report
Fri 10 Feb 2017
SRR3559257.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559257.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7029483
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1155381.643620163815746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT577460.8214828885708949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351230.49965267715989925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210100.29888400043075714No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192060.27322066217387536No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132310.18822152354589947No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT115950.16494811922868297No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98520.14015255460465584No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93260.13266978524594197No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC91010.12946898086246172No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT78410.11154447631497225No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC76650.10904073599722769No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA71490.10170022461111294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA462950.061.255511
AACGCAG614650.047.059926
TATCAAC606850.046.681262
ATCAACG610100.046.15943
TCAACGC622350.045.6812324
CAACGCA628000.045.4248055
ACGCAGA685000.042.105247
CGCAGAG700900.041.2519388
GCAGAGT744850.038.777929
GGTATCA222450.037.9013371
GTACTGG58400.034.368681
GTACATG484250.033.9900361
AGCGCGA10100.033.5751235
ACATGGG474950.033.5011183
TACATGG491350.033.4853862
TACTGGT50600.033.0570452
CATGGGG200800.032.7868464
AGAGTAC723100.031.26134510-11
GCGATCA10700.030.580398
CGCATAG13750.029.719091
Produced by FastQC (version 0.11.5)