FastQCFastQC Report
Fri 10 Feb 2017
SRR3559251.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559251.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547356
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396672.5635341834716767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201581.3027383485119133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181511.1730332257088867No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62530.4041086860425138No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA48480.31330863744348425No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38600.2494577847631702No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT34140.2206344241402754No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33190.2144949190748606No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT32400.20938943591519987No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31670.20467171097019687No Hit
ATAGTAGCCTGTGCAGTAATAAATTCCAGTGTCTTCAGCCCTTAAGTTGT31350.20260366715868874No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG30120.19465462375820433No Hit
GGCTACTATAGTAACTACTATGCTATGGACTACTGGGGTCAAGGAACCTC29640.1915525580409421No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG28750.18580081119018507No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG27630.17856265784990655No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27130.17533133939442508No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27080.17500820754887692No Hit
CTACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCT25940.16764080147037916No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25680.16596051587352878No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC24080.15562029681598805No Hit
GTGCAGGGCCAGAGAAGCCATCCCGTGGTGGGACGAACACATTTACATTG23450.15154883556208137No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22100.1428242757322814No Hit
TCCCAACACTGAGGACAGGGGGCAAGTACCTAGCCACCTCGCAGGTGTTG21610.13965758364590952No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20070.1297051228030266No Hit
GCATAGTAGTTACTATAGTAGCCTGTGCAGTAATAAATTCCAGTGTCTTC19460.12576291428733918No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA18620.12033429928213028No Hit
GTGTTGGGAAGGTTCTGATACCCTGGATGACTTCAGTGTTGTTCTGGTAG18360.11865401368527993No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16890.10915393742616437No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG16690.10786141004397179No Hit
CTATAGTAGCCTGTGCAGTAATAAATTCCAGTGTCTTCAGCCCTTAAGTT16260.1050824761722577No Hit
GTACAATGTCTCCCTGATCATGTCTGACACAGGCGGCACCTGCTATTGAC16080.10391920152828438No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT16050.10372532242095549No Hit
GTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTAC16030.10359606968273624No Hit
GTATGTGACCAGTGCCCCGATGCCAGAGCCTGGGGCCCCAGGCTTCTACT16010.10346681694451698No Hit
CATTTACATTGGGGTTCATCTCTGCGACAGCTGGAATGGGCACATGCAGA15860.10249742140787253No Hit
CTACTATAGTAACTACTATGCTATGGACTACTGGGGTCAAGGAACCTCAG15610.10088176218013178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG1950.085.429362
CGCATAG3550.080.8968051
TAACGCA3200.070.650844
GTACAAC2500.069.402721
GAGTTCT5250.056.980881
ACAACGC3050.056.569443
TCACGCA2000.056.520674
ATAGTAC5150.055.4520654
TATCACG2100.053.8292122
TAGTACT5400.052.8814165
AGTTCTT5800.052.314962
ATAACGC5600.050.9960943
AGATTAC6600.050.4809842
GTATCAC2850.050.38311
AAACGCA3800.048.5326235
ATCACGC2500.045.2165373
GATTACT7550.044.129073
TGAGTAC2000.041.644125
AGTGTAC4600.041.388133
GTATAAC5850.040.909351
Produced by FastQC (version 0.11.5)