FastQCFastQC Report
Fri 10 Feb 2017
SRR3559220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925453
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309153.340526207165572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144911.5658277621878152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108871.1763968564584049No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49550.5354134677828047No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37000.39980420399523264No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35010.3783012211317052No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23310.25187664851699654No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22750.24582555786193355No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.22778034108701362No Hit
GTAATAGACCGCAGAGTCCTCAGATGTCAGGCTGCTGAGTTGCATGTAGG18950.2047645855597205No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG18490.19979404680734733No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.17850717432435792No Hit
GTCTATTACTGTGCAAGTAGTAACTACTACTTTGACTACTGGGGCCAAGG15800.17072720062499122No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.15041282485442264No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT12850.1388509194956416No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12450.13452871188488233No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11660.12599235185363275No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11370.12285875133583228No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT11350.12264264095529433No Hit
TCCTAATAGTGGTAGTACTAACTACAATGAGAAGTTCAAGAGCAAGGCCA11300.12210236500394941No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG10940.11821237815426607No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT10650.11507877763646561No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10440.11280961864081698No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT10310.11140490116732021No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC9950.10751491431763688No Hit
GTCCTGCCCAGTTCCTGTTTCTGTTAGTGCTCTGGATTCGGGAAACCAAC9920.10719074874682992No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC9790.10578603127333318No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9670.10448936899010539No Hit
CTCTTAGATAGTGATGGAAAGACATATTTGAATTGGTTGTTACAGAGGCC9590.10362492746795353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9510.10276048594580169No Hit
CCTATGTTCATTTCCTCAAAATGATGAGTCCTGCCCAGTTCCTGTTTCTG9470.10232826518472575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG2800.079.4738541
TACAACG1800.062.7975732
ATAACGC2600.061.7772673
TCACGCA1750.061.1856084
AGTGTAC4050.060.2236563
AGATTAC5250.057.792652
TATCACG2000.056.517812
GTATCAC2450.056.4601861
AAACGCA2950.054.4448175
TAACGCA2750.054.078194
GTATAAC2800.053.6985471
ATCACGC2000.053.54033
CGAGAGT1006.91216E-1153.5374074
ATAGTAC4600.053.020144
CAGTGTA4800.049.577032
GCATAGT4900.048.565252
CGTATCA1251.0913936E-1148.11391
GTATCAA151300.047.54151
GCAGTGT4900.046.6410261
TCAAACG1152.746674E-1046.5567863
Produced by FastQC (version 0.11.5)