FastQCFastQC Report
Fri 10 Feb 2017
SRR3559217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6482931
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2656054.096989463562084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1481372.2850312613230033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT726561.1207276461834932No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351600.5423472808826748No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228580.352587433060756No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159100.2454136871115858No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145380.2242504200646282No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128640.19842876624785918No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT117520.18127603085703056No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT104710.16151644988971808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG103350.15941863333112755No Hit
GTATCAACGCAGAGTACATGGGCAGGAAACAGCTATGACCCTGTCTCTTA100830.1555315026490333No Hit
GGTCATAGCTGTTTCCTGCCCATGTACTCTGCGTTGATACCTGTCTCTTA98370.15173692269746508No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT75890.11706124899370361No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC73530.11342092025967884No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65580.10115794846497672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154050.097.027241
GTATCAA650950.089.128781
CGTATCA6350.063.5155031
TATCAAC961550.061.3480722
ATCAACG1020300.057.799053
TCAACGC1043850.056.5919344
CAACGCA1069000.055.2716455
AACGCAG1093250.054.18716
ACGCAGA1225600.048.223927
CGCAGAG1232100.047.994398
GGTCATA70350.045.0946661
GCAGAGT1323750.044.6265569
GTCATAG76000.041.724742
AGAGTAC1297250.036.64697610-11
TCATAGC95050.035.6093
AGTATCA22950.034.3611151
TACTGGT67200.034.174182
GGTTCAC64600.033.8855826
AGTACTT1075550.033.80606512-13
GTACTGG72400.033.341451
Produced by FastQC (version 0.11.5)