FastQCFastQC Report
Fri 10 Feb 2017
SRR3559203.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559203.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7277161
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1015641.3956541568889296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490950.6746449611325076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325980.44794941323958615No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA321290.4415045922441458No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT265780.365224845238411No Hit
ATGGTACTCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAACGG251780.3459865736102307No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG237170.3259100630039654No Hit
TCATTATGGTACTCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAA222360.305558719945869No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT215230.29576094303808864No Hit
TTATGGTACTCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAAC214240.2944005224015244No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC202460.2782128909886699No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC199530.2741865955693436No Hit
GACATGAGTGTGCCCACTCAGGTCCTGGGGTTGCTGCTGCTGTGGCTTAC184960.25416505145344453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181100.24886078513310342No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175150.2406845196911268No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT167350.22996605406971204No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA157400.21629313959111254No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA156440.21497394382232302No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG148140.20356839707133043No Hit
CCCTATCTGCATCTGTGGGAGAAACTGTCACCATCACATGTCGAGCAAGT143740.19752208313104522No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT140410.19294612280805662No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT132130.18156805930224712No Hit
GGTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTGGTCTATAATGCA130880.17985035647830244No Hit
GAGTACCATAATGATGTTGACAGTAATAACTCCCAAAATCTTCAGGCTGC130750.1796717153846122No Hit
CCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAACGGGCTGATGC130060.17872354342579477No Hit
ATCTGCATCTGTGGGAGAAACTGTCACCATCACATGTCGAGCAAGTGAGA129730.17827006988027336No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT126190.17340553548286206No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT123310.16944794817649356No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA121970.16760657074922486No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT121600.16709813071333726No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG119700.16448722242094135No Hit
GTACCATAATGATGTTGACAGTAATAACTCCCAAAATCTTCAGGCTGCAG118650.16304435204882783No Hit
GGTCTATAATGCAAAAACCTTAGCAGAAGGTGTGCCATCAAGGTTCAGTG115790.15911424798764245No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT115070.1581248511610503No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA113670.15620102399823227No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC112490.15457951253242852No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112310.15433216332578048No Hit
CCATAATGATGTTGACAGTAATAACTCCCAAAATCTTCAGGCTGCAGGCT109750.1508143079423418No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG108680.14934395432504516No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT108190.14867061481805888No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA107830.1481759164047628No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT106660.1465681465615506No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC106620.14651318007118436No Hit
GTCTCCAGCCTCCCTATCTGCATCTGTGGGAGAAACTGTCACCATCACAT103990.14289913332960477No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC103460.14217082733225225No Hit
CTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTG102120.14032944990498356No Hit
GTTATTTAGCATGGTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTG101720.13977978500132124No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG101390.13932631145579985No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC96020.13194706012413357No Hit
GATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTGGGGAGGAGGT95650.13143862008824597No Hit
GTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACAT94320.12961098428356882No Hit
TATTTACAGTTATTTAGCATGGTATCAGCAGAAACAGGGAAAATCTCCTC93800.12889641990880785No Hit
TCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTCAGTCGTG91780.12612061214531325No Hit
TGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACG90010.12368834494660762No Hit
TTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACT89310.12272643136519859No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG87780.12062396310869032No Hit
GGTCCTGGGGTTGCTGCTGCTGTGGCTTACAGGTGCCAGATGTGACATCC86920.1194421835658164No Hit
ATATTAGCATGATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTG86860.11935973383026705No Hit
TCTCAGGACCTTTGTCTCTAACACTCATTCCTGTTGAAGCTCTTGACAAT86660.1190849013784359No Hit
CAGTTGGACTGATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCA86440.11878258568142164No Hit
GTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTG86360.11867265270068918No Hit
GGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTC86330.1186314278329145No Hit
GGCTTACAGGTGCCAGATGTGACATCCAGATGACTCAGTCTCCAGCCTCC85220.11710610772525164No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83100.1141928837358415No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA83020.11408295075510902No Hit
GGGTAGAAGTTGTTCAAGAAGCACACGACTGAGGCACCTCCAGATGTTAA82890.11390430966141879No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC80950.11123843487865666No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG80740.11094986080423395No Hit
ATTTAGCATGGTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTGGTC78830.1083252108892465No Hit
ATCACATGTCGAGCAAGTGAGAATATTTACAGTTATTTAGCATGGTATCA77630.10667621617825962No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC77030.10585171882276619No Hit
ATGCTGCACCAACTGTATCCATCTTCCCACCATCCAGTGAGCAGTTAACA77010.10582423557758307No Hit
GTGCAGCATCAGCCCGTTTTATTTCCAGCTTGGTCCCCCCTCCGAACGTG75950.104367623582878No Hit
AGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCT75370.10357060947256767No Hit
GGTTTGGAGCACCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAG75000.10306216943668003No Hit
CAGTTTTCTCTGAAGATCAACAGCCTGCAGCCTGAAGATTTTGGGAGTTA74280.10207277261008792No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA72860.10012146220208679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG7250.072.216732
CGCATAG10200.070.351921
TATCACG5900.061.5135772
TAACGCA9800.059.4963264
TCACGCA6950.055.6440474
GTACAAC9500.055.3928871
TAGTACT14350.050.1676335
ATAGTAC14550.049.4780434
AGATTAC17150.046.8341672
ATCACGC8300.044.443043
GTATAAC18200.041.0708351
AGTGTAC13400.039.516223
ATCTACG7450.039.1320842
GTATCAC11650.039.0105971
GAGTTCT18300.037.9054641
AAACGCA14450.037.4682775
GATTACT21600.037.1852043
TCAAACG6250.037.125713
TACTGGT20600.035.524752
CAAACGC7650.034.997844
Produced by FastQC (version 0.11.5)