FastQCFastQC Report
Fri 10 Feb 2017
SRR3559188.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559188.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834591
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT723021.8855205157473118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338730.8833536614465531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242010.6311233714364843No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA158960.41454225496278485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132880.34652978635791926No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCATTCACGTTCGGCTCGGG130190.3395146966130156No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124190.3238676562898103No Hit
GTCTACAGCATGGTGAGAGCCCATTCACGTTCGGCTCGGGGACAAAGTTG114270.29799788295544427No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT112360.29301690845255723No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT109590.28579319150334415No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT106400.27747418173150673No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC99250.25882812534635374No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG98410.25663753970110503No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG87670.22862933752256762No Hit
GTACATGGGGTTGTCATTGCAGTCAGGACTCAGCATGGACATGAGGGCCC80730.21053092754872685No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG80660.2103483787449561No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79050.20614975625822934No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC71970.18768624867684713No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA70760.18453076221166742No Hit
GATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTG69280.18067115893194347No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT67610.17631606604198466No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT65080.16971823070569977No Hit
CCTAAGACCCTGATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATC64700.1687272514852301No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT64640.16857078108199808No Hit
GCTGTAGACAGTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATG63250.16494588340712218No Hit
CTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCA62030.16176431854140377No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT61160.159495497694539No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT60340.15735706885036763No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT59980.15641824643097532No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA58820.15339315196848893No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG58640.15292374075879278No Hit
ATTTAAGCTGGTACCAGCAGAAACCATGGAAATCTCCTAAGACCCTGATC57160.14906413747906883No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA55920.14583041581227307No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54110.1411102253147728No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG53950.140692970906154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53590.1397541484867617No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT53100.1384763068603666No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52750.13756356284151294No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT52680.13738101403774222No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC52550.13704199483073945No Hit
ATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCAGT50690.1321914123305458No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC49820.12992259148368104No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49510.12911416106698212No Hit
CCATGTATGCATCGCTGGGAGAGAGAGTCACTATCACTTGCAAGGCGAGT48070.12535887138941285No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA48070.12535887138941285No Hit
AGATTATTCTCTAACCATCAGCAGCCTGGAGTCTGACGATACAGCAACTT48050.1253067145883355No Hit
GTCTCCATCCTCCATGTATGCATCGCTGGGAGAGAGAGTCACTATCACTT47880.12486338177917801No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA47400.12361161855332159No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC47380.12355946175224425No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG47150.1229596585398547No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC46860.12220338492423312No Hit
GATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTGGGGAGGAGGT46120.12027358328437114No Hit
TCCTCCATGTATGCATCGCTGGGAGAGAGAGTCACTATCACTTGCAAGGC46070.12014319128167776No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC45200.11787437043481301No Hit
GCATGGACATGAGGGCCCCTGCTCAGTTTTTTGGGATCTTGTTGCTCTGG43980.1146928055690946No Hit
TGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACG43970.11466672716855592No Hit
CTACAGCATGGTGAGAGCCCATTCACGTTCGGCTCGGGGACAAAGTTGGA43650.11383221835131829No Hit
ATATTAGCATGATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTG42080.10973790946674626No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG41840.10911202785381804No Hit
TTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACT41540.10832967583765778No Hit
TCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTCAGTCGTG40620.10593046298809963No Hit
GTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACAT40440.1054610517784035No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC40410.10538281657678746No Hit
GTTTTTTGGGATCTTGTTGCTCTGGTTTCCAGGTATCAGATGTGACATCA40310.10512203257140071No Hit
GGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTC40030.1043918373563178No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA39770.10371379894231222No Hit
GGGCAAGATTATTCTCTAACCATCAGCAGCCTGGAGTCTGACGATACAGC39730.10360948534015753No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.10170576210083422No Hit
GGTTTCCAGGTATCAGATGTGACATCAAGATGACCCAGTCTCCATCCTCC38960.10160144849867953No Hit
TCTCAGGACCTTTGTCTCTAACACTCATTCCTGTTGAAGCTCTTGACAAT38710.10094948848521264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA4950.076.9225854
CGCATAG7300.072.845371
TACAACG4350.072.487932
TATCACG5350.070.059312
AGTGTAC9650.064.118763
GTATCAC6950.063.618251
GTACAAC5150.062.6500361
ATCACGC6500.057.66423
AGATTAC10850.057.0272832
GCATAGT9550.055.4454232
ATAGTAC9700.054.588024
TAACGCA6500.054.002984
TAGTACT10400.051.4858935
ACAACGC6800.048.9957243
AAACGCA8050.047.3002245
CATAGTA11350.046.652323
GAGTTCT10550.046.4404261
ATCTACG4800.045.860582
TGTACTT14250.045.5083125
TACGCAG5200.044.621115
Produced by FastQC (version 0.11.5)