FastQCFastQC Report
Fri 10 Feb 2017
SRR3559178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24246154
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260971.757379747732362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2071520.8543705529545016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1394230.5750314049807652No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG814400.33588832274182534No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT631630.26050729530134964No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT562900.23216053152182403No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT522010.21529600117197967No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT516040.21283375499470966No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG489000.20168147080151352No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC443550.1829362298037041No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC423070.17448952934968573No Hit
GTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGT383700.15825190254916305No Hit
CTCTAGGGGAGAAGGTCACCATGAGCTGCAGGGCCAGCTCAAGTGTAAAT374780.15457296856235425No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT374470.15444511323321627No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG374210.15433787973135862No Hit
GATATACACCGACACTGGAGAGCCAACATATGCTGAAGAGTTCAAGGGAC353480.14578807014093864No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA322730.13310564636354286No Hit
GTGCAGGGCCAGAGAAGCCATCCCGTGGTGGGACGAACACATTTACATTG322030.1328169407816184No Hit
CTCTGAAGCCACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAG318820.13149301947022196No Hit
GTTTACTAGTTCCCCATCTACGTTCGGTGCTGGGACCAAGCTGGAGCTGA315840.130263958564315No Hit
GTCTGGACCTGAGCTGAAGAAGCCTGGAGAGACAGTCAAGATCTCCTGCA299470.12351237231273875No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297910.12286897130159283No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296710.12237404744686517No Hit
GTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTAC288780.1191034256402067No Hit
TCCCAACACTGAGGACAGGGGGCAAGTACCTAGCCACCTCGCAGGTGTTG288260.11888895863649138No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT288030.11879409823100191No Hit
GTGCTGGGACCAAGCTGGAGCTGAAACGGGCTGATGCTGCACCAACTGTA284540.11735469468683568No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281330.11603077337543925No Hit
GGTCACATACTTCTCTTGGGGCAAGAGTTGCCCTCTCTGAAGCCACTGCA280570.11571732160077842No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT274850.11335818455991No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT264680.10916370489109326No Hit
CATCCAACCTGGCTCCTGGAGTCCCAGCTCGCTTCAGTGGCAGTGGGTCT261530.10786452977243317No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT261390.1078067886560483No Hit
CTCTTATTCTCTCACAATCAGCAGCATGGAGGGTGAAGATGCTGCCACTT261310.10777379373239977No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC258530.10662722013561408No Hit
GCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAGC257480.1061941617627274No Hit
GTCTTAGACATCATGGATTGGGTGTGGACCTTGCTATTCCTGATAGCAGC252500.10414022776560768No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251980.10392576076189237No Hit
CCCAGGGCCTGCCTGGTTGAGCGCTAGCATGGTCAATAGCAGGTGCCGCC250080.10314213132524028No Hit
CCCCCAAACTATGGATTTATTACACATCCAACCTGGCTCCTGGAGTCCCA249880.10305964401611901No Hit
GTCCAGAGGAGAAAATGTGCTCACCCAGTCTCCAGCAATCATGTCTGCAT243570.10045716941334283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1183500.085.74471
TATCAAC1699400.059.7989232
ATCAACG1718950.058.9896623
TCAACGC1752550.057.9775284
CAACGCA1775550.057.21985
GGTATCA570850.056.2583351
AACGCAG1813650.056.139146
ACGCAGA2035200.050.0268177
GCAGAGT2150500.047.236129
CGCAGAG2239250.045.456978
AGAGTAC2108900.036.8952810-11
GCATACA342850.034.3954242
AGTACTT1508250.034.1050612-13
GTCTATA68200.032.8226431
CATACAC384000.031.8090573
CAGAGTA2123500.031.46092810-11
GACCGTT25650.030.3873717
TACTTTT1702600.030.21256314-15
GAGTACT1465250.030.18244612-13
AGACTCA104200.028.5503527
Produced by FastQC (version 0.11.5)