FastQCFastQC Report
Fri 10 Feb 2017
SRR3559171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099250
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT397181.8920090508514946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201340.9591044420626413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139100.6626176015243539No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82240.3917589615338811No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74780.3562224604025247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74380.35431701798261284No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA67780.32287721805406694No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT62690.2986304632606884No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG55600.2648564963677504No Hit
CTATTACTGTCTACAGTTTTATGAGTTTCCGCTCACGTTCGGTGCTGGGA53050.25270930094081223No Hit
AGTCAGGACTCAGCATGGACATGAGGGCTCCTGCTCAGTTTCTTGGGATC49210.2344170537096582No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46770.22279385494819579No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT45530.216886983446469No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG44690.21288555436465403No Hit
GTACATGGGATTGTAGTCAGGACTCAGCATGGACATGAGGGCTCCTGCTC44540.21217101345718709No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC42130.20069072287721806No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT41400.1972132904608789No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41010.19535548410146483No Hit
ACTCAAGACATTGTTAAGAATTTAAACTGGTATCAGCAGAAACCAGGGAA39980.19044896987019175No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA35790.17048946052161487No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT35160.16748838871025368No Hit
GTGCTGGGACCAAGCTGGAGCTGAAACGGGCTGATGCTGCACCAACTGTA34020.16205787781350484No Hit
CAGTAATAGTCTGCAAAATCTTCAGACTCCAGGTTGCTGATTGTCAGAGA33860.16129570084554007No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC32750.15600809813028463No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30780.14662379421221866No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30590.1457187090627605No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT30480.14519471239728474No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30440.14500416815529357No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT29670.14133619149696322No Hit
GACTATTACTGTCTACAGTTTTATGAGTTTCCGCTCACGTTCGGTGCTGG29010.1381922115041086No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG28930.13781112302012624No Hit
GTATTACTGTGTGAGACATTCGGATTACTACGGTAGTATCGATGTCTGGG28830.13733476241514828No Hit
ATATTATGCCGATTCAGTGAAAGACAGATTCACCATCTCCAGAGATGATT28760.13700130999166368No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT28730.1368584018101703No Hit
CTCTATGTCTGCATCTCTGGGAGACAGAATAACCATCACTTGCCAGGCAA27800.1324282481838752No Hit
GAATAACCATCACTTGCCAGGCAACTCAAGACATTGTTAAGAATTTAAAC27340.13023698940097653No Hit
CTATTATGCAACTGAACTGGCAGAAGGGGTCCCATCAAGGTTCAGTGGCA27050.12885554364654042No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT26940.12833154698106466No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT26830.1278075503155889No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.12723591758961533No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG26460.12604501607717042No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA25890.12332976062879598No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA25820.12299630820531142No Hit
GTCTCCATCCTCTATGTCTGCATCTCTGGGAGACAGAATAACCATCACTT25770.12275812790282242No Hit
GTACATGGGGATTGTAGTCAGGACTCAGCATGGACATGAGGGCTCCTGCT25700.12242467547933786No Hit
GTGCAGGGCCAGAGAAGCCATCCCGTGGTGGGACGAACACATTTACATTG25360.12080504942241276No Hit
GGATTGTAGTCAGGACTCAGCATGGACATGAGGGCTCCTGCTCAGTTTCT25060.11937596760747886No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA24850.11837561033702514No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24540.11689889246159343No Hit
GTAATAGTCTGCAAAATCTTCAGACTCCAGGTTGCTGATTGTCAGAGAAT24380.11613671549362868No Hit
GTTTTATGAGTTTCCGCTCACGTTCGGTGCTGGGACCAAGCTGGAGCTGA24220.11537453852566394No Hit
CTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTG23970.11418363701321901No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC23360.1112778373228534No Hit
GTCTACAGTTTTATGAGTTTCCGCTCACGTTCGGTGCTGGGACCAAGCTG23160.11032511611289747No Hit
ATGTATTACTGTGTGAGACATTCGGATTACTACGGTAGTATCGATGTCTG23130.11018220793140407No Hit
GTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGT22540.10737168036203405No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC22380.10660950339406931No Hit
GCATGGACATGAGGGCTCCTGCTCAGTTTCTTGGGATCTTGTTGCTCTGG22370.10656186733357151No Hit
TCCCAACACTGAGGACAGGGGGCAAGTACCTAGCCACCTCGCAGGTGTTG21940.10451351673216626No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21860.10413242824818389No Hit
GGGTCAGACTATTCTCTGACAATCAGCAACCTGGAGTCTGAAGATTTTGC21740.1035607955222103No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21720.1034655234012147No Hit
GTCCCAGCACCGAACGTGAGCGGAAACTCATAAAACTGTAGACAGTAATA21630.10303679885673456No Hit
GTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTAC21150.1007502679528403No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC21060.10032154340836014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG2800.087.116232
CGCATAG4800.077.103531
TCACGCA2350.070.8864754
ACAACGC3650.066.828893
GTACAAC3850.066.670171
TAACGCA4600.060.787364
AGATTAC6200.059.4940072
TATCACG2750.058.41232
ATAGTAC6700.055.9421274
GTATCAC3650.055.6044921
TAGTACT6800.055.119455
GCATAGT6800.055.119452
ATCACGC3050.052.6668243
GTATAAC6150.052.4134141
AGTGTAC4700.051.8990253
ACGCATA1650.050.648651
AAACGCA5250.049.861655
CATAGTA7850.047.7467843
ATAACGC6900.047.422763
ATCTACG2250.039.6626742
Produced by FastQC (version 0.11.5)