FastQCFastQC Report
Fri 10 Feb 2017
SRR3559170.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559170.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272303
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394621.736652198232366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197780.8703944852425051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141860.6243005444256333No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102690.4519203644936437No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.3679966976235124No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77050.33908330007045717No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75370.3316899198742421No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG65190.2868895565424153No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT64290.28292881715158585No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT55780.24547782580052044No Hit
GTCTGGACCTGAGCTGAAGAAGCCTGGAGAGACAGTCAAGATCTCCTGCA55230.24305737395056906No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47900.21079935202303565No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45870.20186568428594248No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC45050.1982570106187423No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG41370.1820619873317951No Hit
GTCTTAGACATCATGGGTTGGCTGTGGAACTTGCTATTCCTGATGGCAGC36380.16010188782041831No Hit
GTGCAGGGCCAGAGAAGCCATCCCGTGGTGGGACGAACACATTTACATTG35450.15600912378322787No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG35140.15464486910416436No Hit
GTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGT33450.14720748069249567No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG31960.14065025658990019No Hit
GTATCAACAGTGTGGAACCTGAAGATGTTGGAGTGTATTACTGTCAAAAT31000.13642546790634877No Hit
TCCCAACACTGAGGACAGGGGGCAAGTACCTAGCCACCTCGCAGGTGTTG30970.13629344325998777No Hit
CTCTGAAGCCACTGCACACTGATGTCTGCAGGAGAGAAGCCCTTCACCAG30540.13440108999548034No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG29380.1292961370028557No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT28820.12683167693745068No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC28740.1264796112138214No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28700.12630357835200673No Hit
GTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTAC28350.12476329081112864No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28310.12458725794931398No Hit
GTCTTAAGGCACCACTGAGCCCAAGTCTTAGACATCATGGGTTGGCTGTG27910.12282692933116754No Hit
ACATATTTCTGTGCAAGATCGGATTACTACGGTAGTAGCTACGGGTACTA26960.11864614886306975No Hit
CCCAGGGCCTGCCTGGTTGAGCGCTAGCATGGTCAATAGCAGGTGCCGCC26220.11538954091949885No Hit
GGTCACATACTTCTCTTGGGGCAAGAGTTGCCCTCTCTGAAGCCACTGCA25960.11424532731770366No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25780.11345317943953777No Hit
CATTTACATTGGGGTTCATCTCTGCGACAGCTGGAATGGGCACATGCAGA25170.11076867829686446No Hit
GTGTTGGGAAGGTTCTGATACCCTGGATGACTTCAGTGTTGTTCTGGTAG24360.10720401284511792No Hit
CAGTATCAACAGTGTGGAACCTGAAGATGTTGGAGTGTATTACTGTCAAA24210.10654388961331301No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC23290.10249513379157621No Hit
ATGTAAATGTGTTCGTCCCACCACGGGATGGCTTCTCTGGCCCTGCACCA23090.10161496948250301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG3250.078.7213442
TATCACG2700.068.313312
TCACGCA3150.062.3292164
CGCATAG5250.057.99271
GTACAAC4650.057.772611
GTATCAC4700.055.8878331
TAACGCA4350.054.709034
ACAACGC5150.049.6763343
ATAGTAC6250.049.5007324
TAGTACT6850.046.902015
ATCACGC4100.044.9848373
GCATAGT7200.043.7976462
GTCTATA8350.043.611981
AGTGTAC5500.042.188123
GTATAAC7450.041.668661
AGATTAC6350.041.227442
AAACGCA5700.040.707845
ACGCATA2350.038.105341
CATAGTA8050.037.6931653
ACGCGGA1452.584784E-937.0541571
Produced by FastQC (version 0.11.5)