FastQCFastQC Report
Fri 10 Feb 2017
SRR3559169.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559169.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939469
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319851.6491627347485316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158320.816305906410466No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA120010.6187776138726631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114420.5899552918865937No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113540.5854179674952268No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92810.4785330417758675No Hit
GATATGGGTGTACCCACTCAGCTCCTGTTGCTGTGGCTTACAGTCGTAGT91580.4721910997288433No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69890.3603563655825383No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62370.32158286623813015No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT60170.3102395552597128No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC59280.3056506703638986No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG57620.29709162662563826No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG54390.2804375836891438No Hit
GAACACACACAGATATGGGTGTACCCACTCAGCTCCTGTTGCTGTGGCTT49190.2536261213765211No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG48740.2513058986763903No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT48590.25053249110968No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT46410.23929230114015745No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC45800.23614711036886898No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG45210.2331050406064753No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44480.22934112378181862No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA39750.20495300517822146No Hit
GTCTGCATCTGTGGGAGAAACTGTCACCATCACATGTGGAGCAAGTGAGA37710.19443466227096182No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT36300.18716463114388526No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA35540.18324603280588656No Hit
CAGATATGGGTGTACCCACTCAGCTCCTGTTGCTGTGGCTTACAGTCGTA35460.18283354877030775No Hit
GCTTTAAATTGGTATCAGCGGAAACAGGGAAAATCTCCTCAGCTCCTGAT34570.17824466387449348No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG32680.16849972853394407No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT31760.16375616212478777No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT31560.16272495203584073No Hit
GCCTCACACAGAACACACACAGATATGGGTGTACCCACTCAGCTCCTGTT31210.16092033438018344No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC30640.1579813856266844No Hit
TATTTACGGTGCTTTAAATTGGTATCAGCGGAAACAGGGAAAATCTCCTC30480.15715641755552678No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT29020.1496285839062135No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT27770.1431835208502946No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG26720.1377696678833227No Hit
GTATTACTGTCAAAATGTGTTAAGTACTCCGTACACGTTCGGAGGGGGGA26460.13642909476769158No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA26090.13452135610313956No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC26000.1340573115631134No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT25890.13349014601419254No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT25370.13080899978293029No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC25200.12993247120732532No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA25070.12926218464950975No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG24750.1276122485071945No Hit
GTACATGGGAGCCTCACACAGAACACACACAGATATGGGTGTACCCACTC24580.12673571993158952No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.12601387286932658No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA24320.1253951468159584No Hit
GTATTCTCTCAAGATCAGTAGCCTGCATCCTGACGATGTTGCAACGTATT24300.1252920258070637No Hit
GTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGT24000.12374521067364315No Hit
ACGTATTACTGTCAAAATGTGTTAAGTACTCCGTACACGTTCGGAGGGGG23880.12312648462027492No Hit
GATCTATGGTGCAACCAACTTGGCAGATGGCATGTCATCGAGGTTCAGTG23820.12281712159359082No Hit
ATATATTACTGTGCCAGAAATTATGATTACGACTGGTACTTCGATGTCTG23810.12276556108914347No Hit
GTAATACGTTGCAACATCGTCAGGATGCAGGCTACTGATCTTGAGAGAAT23770.12255931907135408No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA23450.12090938292903881No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC23420.12075470141569677No Hit
GTGTTAAGTACTCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAA22510.1160626955109878No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22220.1145674408820146No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC22100.11394871482864641No Hit
CTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTG21570.11121600809293679No Hit
GTAATATATGGCTGTGTCATCAGCTTGCAGACTGTTCATTTTAAAGAAAA20870.10760677278162217No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC20420.10528655008149136No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG20390.10513186856814932No Hit
TTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACT20280.10456470301922846No Hit
GTTAAGTACTCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAAC20050.10337881141693939No Hit
GTCAATAGCAGGTGCCGCCTGTGTCAGACATGATCAGGGAGACATTGTAC20020.10322412990359732No Hit
CCCAGGGCCTGCCTGGTTGAGCGCTAGCATGGTCAATAGCAGGTGCCGCC19700.10157419376128209No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC19660.10136795174349267No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT19510.1005945441767824No Hit
CAGTTGGACTGATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCA19480.10043986266344035No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19460.10033674165454566No Hit
CATGTGGAGCAAGTGAGAATATTTACGGTGCTTTAAATTGGTATCAGCGG19450.10028518115009831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2200.078.429362
TACAACG3050.078.0303342
CGCATAG3700.075.826991
TAACGCA3600.071.0653844
TCACGCA2500.069.016054
GTACAAC3800.062.835351
TAGTACT4600.060.7900055
ATCACGC3100.059.4965933
GTATAAC4650.059.0517161
ATAGTAC4850.057.6564984
GCATAGT5000.055.9282462
GTATCAC3600.053.0609631
AGATTAC6400.052.9905242
TCTAACG453.1719657E-452.887232
ACGCATA1350.048.6392141
GATTACT7550.045.7059943
ACAACGC5250.044.197473
CGCAAAG2450.043.856511
TCAAACG2050.043.53413
GAGTTCT5350.043.5149461
Produced by FastQC (version 0.11.5)