FastQCFastQC Report
Fri 10 Feb 2017
SRR3559162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625993
Sequences flagged as poor quality0
Sequence length125
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1046996.439080611048141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT646753.977569399130255No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT536043.2966931591956428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332932.0475487901854437No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG214541.319439874587406No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208151.2801408124143214No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153660.9450225185471278No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145210.8930542751414059No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124360.7648249408207785No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG121860.7494497208782571No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120400.7404705924318247No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT101540.6244799331854443No Hit
GTGATAAGGTACCCGGGGATCCTCTAGAGTCGACCTGCCCATGTACTCTG85030.5229419806850337No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT80240.49348305927516295No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73610.4527079759875965No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT68460.4210350229060027No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT63830.3925601155724533No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT63440.39016158126142No Hit
CCTTATCACATCGCGCGCTGGAAGATGCGATGTGATAAGGTACCCGGGGA62730.3857950187977439No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49660.30541336893824267No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGGTCGACTC42130.2591032064713686No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41320.25412163520999165No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA41080.2526456140955096No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40700.25030858066424644No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA39550.24323597949068662No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT38620.2375163976720687RNA PCR Primer, Index 39 (95% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC37490.2305667982580491No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.22380170148333972No Hit
GTATCAACGCAGAGTACATGGGCAGGTCGACTCTAGAGGATCCCCGGGTA35990.22134166629253632No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC34710.2134695536819654No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG34250.2106405132125415No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT34100.20971800001599022No Hit
CTCTAGAGTCGACCTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCA33230.20436742347599282No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32560.20024686453139715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC31810.19563429854864076No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30530.18776218593806984No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT28440.17490850206612207No Hit
ACTCTAGAGGATCCCCGGGTACCTTATCACATCGCGCGCTGGAAGATGCG27870.1714029519192272No Hit
GTACATGGGCAGGTCGACTCTAGAGGATCCCCGGGTACCTTATCACATCG25100.15436720822291364No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC24900.15313719062751194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT24420.15018514839854785No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT24130.14840162288521536RNA PCR Primer, Index 39 (96% over 25bp)
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.14643359473257264No Hit
TATCAACGCAGAGTACATGGGCAGGTCGACTCTAGAGGATCCCCGGGTAC23610.14520357713717094No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC22970.14126752083188548No Hit
GTGATAAGGTACCCGGGGATCCTCTAGAGTCTGTCTCTTATACACATCTC22050.13560943989303767No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT21920.13480992845602657No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT21420.1317348844675223No Hit
ACTCTAGAGGATCCCCGGGTACCTTATCACCTGTCTCTTATACACATCTC21050.12945935191602914No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20410.1255232956107437No Hit
GTACATGGGCAGGTCGACTCTAGAGGATCCCCGGGTACCTTATCACCTGT20290.1247852850535027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC20140.12386277185695142No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA19810.12183324282453861No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT19410.11937320763373521No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA19010.11691317244293178No Hit
GTGATAAGGTACCCGGGGATCCTCTAGAGTCGACCTGCCCATGTACCTGT18890.11617516188569077No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18520.11389962933419763No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC18260.11230060646017541No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT18150.11162409678270448No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCT18060.1110705888647737No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.10762653959764895No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT17410.10707303167971818No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG17340.10664252552132758RNA PCR Primer, Index 39 (96% over 30bp)
GTACCCGGGGATCCTCTAGAGTCGACCTGCCCATGTACTCTGCGTTGATA17010.10461299648891478No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC16770.10313697537443273No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC16760.10307547449466266No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16660.1024604656969618No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGG16350.10055393842408916No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC16330.10043093666454898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCA7550.080.654511
TACTTTG8650.060.529962
AAAAGTA17400.053.3432122
AGTACTC17050.049.202285
GTGTACT1900.046.970824
GTATCAA606350.046.7085881
AGTGTAC1800.046.2763753
ACTTTGC11200.046.217353
ATGGTAT4100.045.1391371
CTTTGCG12150.043.5817074
AAAAAGT22300.041.2279051
TATCAAC751400.037.722842
GCCTAGT801.17424854E-437.3125951
AAGTACT25650.037.1127474
AACGCAG766150.037.0653536
ATCAACG778100.036.3748783
CAACGCA787350.036.0522235
TCAACGC787200.036.0515374
ATAAGGT73050.035.9991764
TAAGGTA72650.035.9516945
Produced by FastQC (version 0.11.5)