FastQCFastQC Report
Fri 10 Feb 2017
SRR3559156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756521
Sequences flagged as poor quality0
Sequence length125
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25256214.378535753344252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1156446.583695839673992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT845994.816281729623499No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT497492.832246241291735No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272621.5520452075437754No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184221.0487776690401083No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181381.0326093454049226No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166130.9457900019413373No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100470.5719829139532064No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73180.41661898719115803No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60560.3447724223052272No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57280.32609914712092825No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCT52780.3004803244595425No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA49930.2842550701073315No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA49030.27913130557505433No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT48140.2740644717598025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC47340.26951001439777833No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42860.2440050531704432No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG35030.1994283017396319No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT31420.178876312893498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC29480.16783175379058946No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA27470.15638867966850384No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAACTGTCTCTTAT24490.1394233259949639No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT24430.1390817416928121No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24210.13782926591825545No Hit
TTTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTAT23110.13156688704547226No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC22190.1263292610791445No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC21870.12450747813433487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT21800.12410896311515775No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC21520.1225149030384493No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATACA20990.11949757503610829No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC20530.11687876205294444No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA20440.11636638559971671No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20330.1157401477124384No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACAT20120.11454460265490705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC19550.11129955178446485No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATAC19370.11027479887800944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG18770.10685895585649133No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT18630.10606192581813711No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGCTGTCTCTTATACACATCTC17800.10133667630503707No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATA17780.10122281487098646No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATC17670.10059657698370814No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC17590.10014113124750572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA733050.069.2714541
CAGGGTA2500.062.0790141
CAGTATC3150.058.743881
GGTATCA306050.058.3358461
AGTATCA6700.055.2367821
TATCAAC918850.055.2337072
GCCTAGT655.3629265E-755.099721
ATCAACG952900.053.2053683
TACGTTG1350.052.8868034
TCAACGC965800.052.6041074
ACCTCTG5550.052.529462
AACGCAG973650.052.192216
CAACGCA985550.051.537875
GAGGTAA1101.7826096E-1048.681283
AGTACTC9050.046.6777155
ACGCAGA1090250.046.6049047
CGCAGAG1096800.046.325118
CGTATCA1550.046.0626952
GCAGAGT1129550.044.8660779
TAGGACC705.4544238E-542.498324
Produced by FastQC (version 0.11.5)