FastQCFastQC Report
Fri 10 Feb 2017
SRR3559140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369484
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT726215.302800178753458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320302.338837109451443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244381.7844677265305764No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122290.8929640652975865No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96930.7077848299067385No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70660.5159607560219761No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54650.3990554106510189No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49420.360865844361818No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC19300.1409289922335712No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19130.13968764877866408No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16470.1202642747195294No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT16440.12004521410983991No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC15800.11537192110313083No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.10763177956076887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA222000.068.6994551
CGCATAG2100.059.6452031
GGTATCA92650.057.5529561
TATCAAC284050.053.835152
AACGCAG295650.052.6026656
ATCAACG291900.052.3408783
TCAACGC299550.051.3418244
CAACGCA304500.050.663515
ACGCAGA328950.047.1329467
CGCAGAG332650.046.6623468
GCAGAGT345550.044.8342749
TACAACG2900.041.035492
GGACCGT4800.038.4238176
GTACAAC3900.038.2341081
TAACGCA2350.037.9754944
TATCACG3000.037.6842582
AAACGCA3800.037.5757525
TCACGCA3350.037.2953344
TACTGGT8300.037.2780272
TAGTACT3550.036.8701065
Produced by FastQC (version 0.11.5)