FastQCFastQC Report
Fri 10 Feb 2017
SRR3559138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5548639
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2034513.6666829469352757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT981701.769262696672103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT637681.1492547992399578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347320.6259553018316744No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238870.43050196633805154No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181060.32631425472084236No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156520.28208719291343337No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138380.2493944911535964No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC115800.20869982711075635No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT110940.19994092244963135No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC83620.15070362299655826No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA83150.14985656843056466No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA78870.1421429651487509No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT71700.12922087740795535No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT63270.11402796253279408No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG57720.10402550967904022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA717850.062.4148981
TATCAAC903300.049.6048662
AACGCAG933300.048.709316
ATCAACG928550.048.099463
TCAACGC949700.047.4730644
CAACGCA960900.046.9630745
ACGCAGA1036150.043.811187
CGCAGAG1041650.043.6446468
GACCGTT13800.042.250327
GGTATCA340500.041.9060131
GCAGAGT1093650.041.5205049
TACAACG8000.040.16662
GTACTGG50600.034.8812451
TACTGGT47100.034.2381062
CGCATAG11150.033.1564181
TTAGGAC18250.032.59993
GGTTCAC48200.032.463236
GGACCGT24200.031.960316
AGAGTAC1078900.031.80323810-11
ACTGGTT52000.030.6627123
Produced by FastQC (version 0.11.5)