FastQCFastQC Report
Fri 10 Feb 2017
SRR3559137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7079704
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1056221.4918985313510282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT497730.7030378671198683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331850.4687342860661971No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192760.27227127009829794No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147320.20808779576095274No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108860.15376349067701137No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81080.11452456204383687No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73570.10391677392162157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA423100.060.3433951
ATCAACG540900.046.944843
TCAACGC549250.046.8160864
AACGCAG558400.046.806426
TATCAAC546400.046.598982
CAACGCA559300.046.0174035
ACGCAGA624050.041.8442547
CGCAGAG637550.041.098488
GGTATCA206750.038.8461721
GCAGAGT679450.038.546529
CGCATAG11950.034.4276471
GTACATG454750.030.379421
ACATGGG435800.030.1160963
AGAGTAC653800.030.08504110-11
TACATGG455950.030.0553342
CATGGGG194350.028.4082094
AGTACTT449650.028.1639312-13
TATCACG8800.027.047192
GAGTACT413900.026.7728812-13
TAACGCA10600.026.3798564
Produced by FastQC (version 0.11.5)