FastQCFastQC Report
Fri 10 Feb 2017
SRR3559136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870793
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222482.554912591166902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106481.222793476750502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82980.9529245182264902No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51750.5942858980262817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45360.5209045088786888No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29220.33555621140730346No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28480.32705821016016434No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21460.24644203616703397No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.188219243838662No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14630.16800778141303388No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12770.14664794044049503No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT12150.13952799344964878No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11580.13298223573225784No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10020.11506753040045109No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC9770.11219658403317435No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT9260.10633985344392985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1700.069.9970252
CGCATAG2650.067.506811
TACAACG2200.051.3841862
GTATAAC2850.050.2155951
GTATCAC2750.049.873221
ATCACGC2650.044.9011733
GTATCAA118800.044.5224841
TCACGCA2550.044.3289074
ACAACGC2750.043.2684023
TAACGCA3200.042.7613534
GCATAGT4350.042.4004972
AGATTAC4500.042.3093152
ACGCATA1151.39807526E-841.4824521
TACGCAG1603.6379788E-1240.9021645
TAGTACT4450.040.1083535
ATAGTAC4500.039.6627044
GTACAAC3250.038.530811
CATGGGG27950.037.676024
AACGCAG147000.037.5198336
TATCAAC142200.037.3219032
Produced by FastQC (version 0.11.5)