FastQCFastQC Report
Fri 10 Feb 2017
SRR3559135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5896658
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1145831.9431854450436163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT553300.9383281173844574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366370.6213180415075794No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197010.3341045046193963No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127470.21617329680642833No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101190.17160567901343438No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86990.1475242416975853No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72000.12210306244655872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA406700.070.1581041
GGTATCA167500.055.002671
TATCAAC543600.052.430742
ATCAACG550550.051.7194983
TCAACGC558600.051.3256384
AACGCAG570550.050.626036
CAACGCA566750.050.608565
ACGCAGA635800.045.3555957
CGCAGAG647750.044.5100448
GCAGAGT682300.042.2997679
GTACATG416400.033.0661281
AGAGTAC670100.033.00160210-11
TACATGG422650.032.4503942
ACATGGG414200.032.1173743
CATGGGT79850.030.9208584
AGTACTT477000.030.18415512-13
TATCACG8000.029.0071162
CATGGGG189450.028.9230044
CAGAGTA678450.028.3423310-11
GAGTACT446200.028.16755912-13
Produced by FastQC (version 0.11.5)