FastQCFastQC Report
Fri 10 Feb 2017
SRR3559133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955273
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT847842.1435688509996655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402491.017603588930524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269350.6809896560869503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151940.384145418028035No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128580.3250850194158532No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87790.22195686618850327No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65380.16529832454042995No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61960.15665163946963964No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56510.14287256530712292No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47240.11943549787840181No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT44380.11220464428119122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA387900.053.910111
CGCATAG9200.046.6658171
AACGCAG498650.043.2279786
TATCAAC483800.043.1304742
ATCAACG489950.042.5140763
TCAACGC499900.042.3343734
CAACGCA506000.041.8710485
ACGCAGA549800.039.1738477
CGCAGAG557950.038.718938
GCAGAGT586950.036.734959
TAACGCA7800.035.8505334
CATGGGT59700.035.6780474
TACAACG7200.034.7081222
ATAGTAC13400.032.4123424
GGTATCA210100.030.0838931
ACATGGG359000.029.549443
TAGTACT14650.029.2406675
AGATTAC17800.029.0812852
GTACATG383500.029.0757181
CATGGGG120700.028.885694