FastQCFastQC Report
Fri 10 Feb 2017
SRR3559130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4501079
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT886121.9686835089986203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424950.9441069574650879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333440.7408001503639461No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265160.5891031905905229No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239100.5312059619482351No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187080.4156336736147044No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141050.3133693054487602No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA86210.19153185269576473No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71380.15858419725581355No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66900.1486310282490043No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54290.12061552352224877No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45110.1002204138163316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG12950.057.5403821
TATCACG8250.051.241112
TAACGCA11250.048.6910864
TACAACG9500.045.752352
GTATCAA452400.045.3085251
TCACGCA10000.044.655554
TAGTACT20050.040.65075
ATAGTAC19600.039.187524
AGATTAC23000.037.536552
TATCAAC556100.036.842232
TCAACGC571150.036.496914
ATCAACG560450.036.4712873
AACGCAG599050.036.139796
CAACGCA578950.036.058215
ATCACGC11900.036.0246433
GCATAGT21700.035.395182
GTATAAC16650.035.0088841
AAACGCA16800.033.9956865
ACAACGC13050.033.7625583
ATAACGC17850.033.356153