FastQCFastQC Report
Fri 10 Feb 2017
SRR3559128.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559128.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228718
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540562.425430224909567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300811.3496996928278948No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241541.08376205513663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229941.0317141962329914No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168910.757879642018416No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127600.5725264479400265No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA118300.5307984231293505No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108220.4855706284958438No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT82840.37169350272219276No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC63000.28267371645941747No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT59330.26620685075455935No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG59230.26575816231573485No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC50640.2272158254207127No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT49460.22192130184258393No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48020.21546018832351155No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46810.2100310582137354No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG45880.20585825573266786No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45260.2030763874119561No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44680.20047399446677416No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT41990.18840427546239585No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC38540.17292452432295158No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT36140.16215600179116424No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG33960.15237459382479074No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA31620.14187528435629812No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC31620.14187528435629812No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC31010.13913828487946883No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT30390.1363564165587571No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC28370.12729291009450278No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.12446617292990858No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA27590.12379314027167188No Hit
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26820.12033823929272344No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC26100.11710768253318724No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25910.11625517449942073No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.11589622374836117No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT25090.11257592930106006No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25060.11244132276941274No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG24530.11006327404364304No Hit
GTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24050.10790956953728556No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA23980.10759548763010843No Hit
CTGCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT23600.10589047156257544RNA PCR Primer, Index 31 (95% over 22bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA23020.10328807861739349No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC22560.10122411179880092No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT22360.10032673492115199No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10400.079.167311
GTATAAC7150.074.8070761
TACAACG5900.074.674572
ATAGTAC11900.069.043884
GCATAGT13400.060.870772
GTACAAC8100.059.3558651
TAACGCA9150.059.212494
TAGTACT14400.058.66355
ACAACGC7850.057.641723
CATAGTA14700.056.2976763
ATAACGC10100.055.411453
AGATTAC16350.053.165282
GGTTTCA13150.052.5570261
TATCACG4950.050.516952
AGTGTAC10550.047.4045333
TCACGCA5350.046.7399834
GTTTCAA15150.045.1937332
GACCGTT4300.044.2734227
GAGTTCT14600.043.632661
GTATCAC8500.043.1290251
Produced by FastQC (version 0.11.5)