FastQCFastQC Report
Fri 10 Feb 2017
SRR3559126.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559126.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7828999
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1151481.4707882834063462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT607260.7756547165225082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441770.5642739256959926No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339290.43337596543312884No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239600.3060416791469765No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA207200.2646570781271016No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174980.22350239155733703No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA125540.1603525559270093No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101120.12916082886202948No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT100220.12801125661147741No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89990.11494445203020208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG11800.047.420342
CGCATAG19600.046.2742921
GTATCAA658250.044.456511
TACAACG15400.042.519762
TAACGCA18400.041.734074
GTATAAC19700.040.8561551
ACAACGC18550.036.262143
AACGCAG876800.035.643836
CAACGCA845200.035.5743185
TCAACGC838200.035.573144
ATCAACG819750.035.4224973
TATCAAC828450.035.107992
TAGTACT26850.034.7869385
AGATTAC39000.034.0376132
ATAGTAC28000.032.7402154
ACGCAGA960550.032.4617277
CGCAGAG975850.032.0686078
GTACAAC25200.031.2240831
GATTACT42450.030.9908473
GCAGAGT1015450.030.6895089