FastQCFastQC Report
Fri 10 Feb 2017
SRR3559119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093008
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378311.8074942857361271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178270.851740652687424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137560.6572359016305719No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100890.4820335134887206No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93990.4490666065299321No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80070.3825594551000283No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT79370.3792149862781222No Hit
GTATTACTGTATGCAAAGTATGGAAGATCCTCGGACGTTCGGTGGAGGCA77160.36865602042610446No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70270.3357368915933432No Hit
CAGTAATACATTGCAGCATCATCTTCCTCCACAGGATCGATGGTGAGAGT64800.3096022566564485No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC63100.30147997523181946No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA51020.24376399899092596No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49650.2372183957251955No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48770.2330139206347993No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG48430.23138946434987348No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG44180.21108376078830088No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT44150.21094042641021915No Hit
GTAATACATTGCAGCATCATCTTCCTCCACAGGATCGATGGTGAGAGTGA43110.20597150130338726No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC42990.20539816379106052No Hit
CTCTAGGACAGAGGGCCACCATATCCTGCCAAGCCAGCGAAAGTGTCAGT42870.20482482627873377No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT42290.20205369496915446No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT41330.19746699487054037No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT41320.19741921674451315No Hit
GTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGGGACCAAGCTG40670.19431363855274322No Hit
ATGTATTACTGTATGCAAAGTATGGAAGATCCTCGGACGTTCGGTGGAGG40640.19417030417466155No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT39450.18848470717742122No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT37970.1814135445253912No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGG37790.18055353825690107No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT36980.17668351004869548No Hit
AAATCAAACGGGCTGATGCTGCACCAACTGTATCCATCTTCCCACCATCC35530.16975568177474715No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT34270.16373563789531623No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC33190.15857560028437542No Hit
GTGGAGGCACCAAGCTGGAAATAAAACGGGCTGATGCTGCACCAACTGTA32570.15561335647068716No Hit
GTATGGAAGATCCTCGGACGTTCGGTGGAGGCACCAAGCTGGAAATCAAA32410.15484890645425148No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC31550.15073998761590973No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA31350.14978442509536513No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31130.1487333063227661No Hit
GTATCCATCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTC31020.14820774693646657No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA30730.1468221812816769No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG29870.14271326244333513No Hit
TGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACG28620.13674099668993142No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA28210.134782093522815No Hit
TTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACT28060.13406542163240656No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC27990.13373097475021598No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.13072095281050047No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA27220.13005205904611927No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.1288098277694113No Hit
GTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACAT26440.12632536521599536No Hit
GATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTGGGGAGGAGGT25790.12321978702422541No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG25700.12278978388998035No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG25460.12164310886532684No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA25320.12097421510094562No Hit
TCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTCAGTCGTG25060.11973198382423766No Hit
GTGCAAAGACTCACTTTATTGAATATTTTCTGCAAATATTAGCATGATAA24640.117725302531094No Hit
GGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTC24330.11624418062424989No Hit
GTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTG24270.1159575118680865No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23370.11165748052563583No Hit
GTACATGGGGTTGTCATTGCAGTCAGGACTCAGCATGGACATGAGGGCCC23050.11012858049276447No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT22950.10965079923249219No Hit
GGGTAGAAGTTGTTCAAGAAGCACACGACTGAGGCACCTCCAGATGTTAA22600.10797856482153916No Hit
ATATTAGCATGATAAAAGCCAAGGAAAGGGAGGAGGAGGAGAAGGAGGTG21730.10382186785717017No Hit
CAGTTGGACTGATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCA21320.10186296469005374No Hit
CTTCCATACTTTGCATACAGTAATACATTGCAGCATCATCTTCCTCCACA21170.1011462927996453No Hit
GTGGAGGCACCAAGCTGGAAATCAAACGGGCTGATGCTGCACCAACTGTA21170.1011462927996453No Hit
TCTCAGGACCTTTGTCTCTAACACTCATTCCTGTTGAAGCTCTTGACAAT20990.10028628653115515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG2900.088.298832
GTACAAC3550.077.685891
CGCATAG4950.076.304131
TAACGCA4750.073.967834
TATCACG2150.069.244622
ATAGTAC5650.068.509284
TAGTACT6200.063.3272175
AGTGTAC5150.062.441173
ACAACGC4200.062.3861053
TCACGCA2500.061.932394
GTATAAC5850.061.490511
GCATAGT6300.061.4408652
CATAGTA6400.060.4808463
GAGTTCT6450.057.629481
AGTTCTT7300.050.5770342
GTATCAC3500.049.6755451
AGATTAC7350.048.6125532
ACGCGTG505.3383893E-447.5913627
TATCTAC2650.047.510121
GTTTCAA8800.045.339492
Produced by FastQC (version 0.11.5)